LOCUS       40924_4749        3420 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pARHS3 DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3420)
  AUTHORS   Okamoto S, Niki H.
  TITLE     pARHS vector sequence
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3420)
  AUTHORS   Okamoto S, Niki H.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAR-2016) Contact:Sho Okamoto National Institute of 
            Genetics, Microbial Genetics Laboratory, Genetics Strains Research 
            Center; 1111 Yata, Mishima, Shizuoka 411-8540, Japan URL 
            :https://www.nig.ac.jp/labs/MicroGen/
REFERENCE   3  (bases 1 to 3420)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3420)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (30-MAR-2016) Contact:Sho Okamoto National Institute of Genetics, 
            Microbial Genetics Laboratory, Genetics Strains Research Center; 
            1111 Yata, Mishima, Shizuoka 411-8540, Japan URL 
            :https://www.nig.ac.jp/labs/MicroGen/"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3420
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        24..42
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             complement(66..88)
                     /label=RBS
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             346..558
                     /codon_start=1
                     /gene="parB"
                     /product="plasmid partition parB protein"
                     /label=parB
                     /protein_id="BAV00617.1"
                     /translation="MSSPHQDSLLPRFAQGEEGHETTTKFPCLVCVDRVSHTVDIHLSD
                     TKIAVRDSLQRRTQGGGGFHGLRIR"
     gene            346..558
                     /gene="parB"
                     /label=parB
     misc_feature    1061..1203
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(1389..1977)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2151..3008)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3009..3113)
                     /label=AmpR promoter
ORIGIN
        1 tcgagatctc gatcccgcga aattaatacg actcactata ggggatccgc tagcccatgg
       61 gtatatctcc ttcttaaagt taaacaaaat tatttctaga aacaaactcc gggaggcagc
      121 gtgatgcggc aacaatcaca cggatttccc gtgaacggtc tgaatgagcg gattattttc
      181 agggaaagtg agtgtggtca gcgtgcaggt atatgggcta tgatgtgccc ggcgcttgag
      241 gctttctgcc tcatgacgtg aaggtggttt gttgccgtgt tgtgtggcag aaagaagata
      301 gccccgtagt aagttaattt tcattaacca ccacgaggca tccctatgtc tagtccacat
      361 caggatagcc tcttaccgcg ctttgcgcaa ggagaagaag gccatgaaac taccacgaag
      421 ttcccttgtc tggtgtgtgt tgatcgtgtg tctcacactg ttgatattca cttatctgac
      481 acgaaaatcg ctgtgcgaga ttcgttacag agacggacac agggaggtgg cggctttcat
      541 ggcttacgaa tccggtaagt agcaacctgg aggcgggcgc aggcccgcct tttcaggact
      601 gatgctggtc tgactactga agcgccttta taaaggggct gctggttcgc cggtagcccc
      661 tttctccttg ctgatgttgt tcctctacgc cggacgcatc gtggccggca tcaccggcgc
      721 cacaggtgcg gttgctggcg cctatatcgc cgacatcacc gatggggaag atcgggctcg
      781 ccacttcggg ctcatgagcg cttgtttcgg cgtgggtatg gtggcaggcc ccgtggccgg
      841 gggactgttg ggcgccatct ccttgcatgc accattcctt gcggcggcgg tgctcaacgg
      901 cctcaaccta ctactgggct gcttcctaat gcaggagtcg cataagggag agcgtcgaga
      961 acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag
     1021 cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg cgcagccatg acccagtcac
     1081 gtagcgatag cggagtgtat actggcttaa ctatgcggca tcagagcaga ttgtactgag
     1141 agtgcaccat atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc
     1201 aggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     1261 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     1321 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     1381 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     1441 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     1501 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     1561 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     1621 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     1681 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     1741 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     1801 tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag
     1861 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     1921 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     1981 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     2041 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     2101 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     2161 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     2221 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     2281 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     2341 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     2401 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     2461 gctgcaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     2521 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     2581 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     2641 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     2701 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     2761 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     2821 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     2881 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     2941 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     3001 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     3061 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     3121 ccccgaaaag tgccacctgg ccttactggt tagcagaatg aatcaccgat acgcgagcga
     3181 acgtgaagcg actgctgctg caaaacgtct gcgacctgag caacaacatg aatggtcttc
     3241 ggtttccgtg tttcgtaaag tctggaaacg cggaagtcag cgccctgcac cattatgttc
     3301 cggatctgca tcgcaggatg ctgctggcta ccctgtggaa cacctacatc tgtattaacg
     3361 aagcgctggc attgaccctg agtgattttt ctctggtccc gccgcatcca taccgccagt
//
